Vitamins and Cofactors Meatbolism Analysis

Precision Metabolomics Services


Vitamins and cofactors are essential regulators of metabolism, enzyme function, and redox balance. They act as biochemical catalysts, controlling cellular energy production, macromolecule synthesis, and detoxification processes. Imbalances in vitamin and cofactor levels can disrupt metabolic pathways, affect immune function, and influence disease development.

Our targeted vitamin and cofactor analyses quantify both water- and fat-soluble compounds, including B vitamins, vitamin D, retinoids, tocopherols, and related metabolites. These data provide insight into nutritional status, enzymatic activity, oxidative stress, and metabolic health across human, animal, and microbial systems.

If you’d like to learn more, we are happy to discuss your project and help you get the most out of your metabolomics analysis.


Let’s discuss your project


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Guiding you from sample to biology

Our metabolomics specialists work with you from the initial planning stage. We begin with a consultation to understand your biological questions, study design, and sample types. Based on your research aims, we help select the appropriate vitamin and cofactor panels and identify possible combinations with other metabolite classes.



Get a Project Estimate

We provide tailored project estimates and timelines according to your experimental scope and setup. Request a free initial consultation to discuss your analytical goals and discover how Biogenity can support your metabolomics research.


Sample preparation and sample analysis

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Vitamins and cofactors can be highly labile and sensitive to light, oxidation, and temperature. We use validated Standard Operating Procedures (SOPs) optimized for compound stability and extraction efficiency to ensure reproducibility and accuracy.

Supported matrices include

  • Biological fluids: plasma, serum, urine, cerebrospinal fluid
  • Cells and tissues: tissue biopsies, cell cultures, microbial and plant extracts
  • Specialized matrices: food, feed, and fermentation samples
  • Model systems: nutritional, clinical, and environmental studies

All workflows are adapted to maintain compound integrity and to minimize degradation or conversion during processing.

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Our vitamin and cofactor analyses employ targeted LC-MS/MS and GC-MS/MS platforms to deliver precise quantification of essential metabolites. High-resolution mass spectrometry ensures excellent sensitivity and reproducibility across a broad dynamic range.


Our Vitamin and cofactors metabolism panels

B Vitamins and Related Cofactors

Quantifies a wide range of B vitamins and their derivatives, including folate, riboflavin, pyridoxal phosphate, niacin, and cobalamin. Reflects cofactor-dependent enzymatic activity and one-carbon metabolism.



Fat-Soluble Vitamins

Includes vitamins A, D, E, and K, as well as their active metabolites. Provides insights into lipid-soluble antioxidant status, hormonal signaling, and nutritional balance.

Redox and Methylation Cofactors

Targets metabolites such as glutathione, SAM, SAH, and related redox cofactors. Enables evaluation of oxidative stress, methylation capacity, and cellular redox regulation.



Vitamin Derivatives and Precursors

Covers biosynthetic precursors and degradation products of essential vitamins. Useful for assessing pathway fluxes and metabolic deficiencies.

Vitamins and cofactors metabolism combo options

Core Metabolomics Combo

Combines Amino Acids and Derivatives, Energy Metabolism, and Lipid Profiling panels for a comprehensive baseline of central metabolism.



Regulatory Metabolomics Combo

Integrates Amino Acids and Derivatives, Energy Metabolism, and Lipid Mediators panels to study signaling and regulation.

Microbiome-Linked Combo

Includes Short-Chain Fatty Acids, Bile Acids, and Indoles to connect host metabolism with microbial activity.



Environmental & Impact Combo

Combines Fatty Acids, Bile Acids, and PFAS analysis to assess how environmental factors influence metabolism.

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Data analysis, including report, figures, and tables

!! Advanced data analysis by metabolomics experts

At Biogenity, our experts manage the complete data analysis workflow for your microbiome metabolite studies, allowing you to focus on interpreting biological mechanisms. We handle every step from data filtering and normalization to advanced statistical evaluation and pathway analysis. Our team ensures your data are accurate, reproducible, and publication-ready.

!! Pathway interpretation and visualization

Reports include quantitative results, QC summaries, and interactive pathway visualizations. These tools highlight microbial pathways and metabolites that differ between conditions, helping you understand how microbial activity and host metabolism are interconnected.

Delivery and discussion

Delivery, post-consultation and discussion

At the conclusion of our lipidomics analysis, we ensure a seamless transition from discovery to application. We deliver detailed reports and data, followed by a post-delivery consultation to discuss your study's insights and findings.


What to expect during post-consultation

  • Explanation of the different steps in the sample analysis
  • Discussion of the data
  • Explanation of the different steps in the data analysis
  • Q&A to the report(s)

Together, we ensure that you have the best prerequisite for your further research. The consultation can also be used to explore the next step, whether you are looking to expand the project with additional omics studies or validate the results.


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