Proteome analysis

Phosphoproteomics is excellent for measuring cell signalling pathways. By applying mass spectrometry to your phosphorylated peptides, Biogenity can detect which proteins, kinases, and pathways are active, thus, identifying which proteins are involved in the biological processes pertaining to your experimental question.

If you’d like to learn more, we are happy to discuss your project and help you get the most out of your phosphoprotein samples.

Let’s discuss your project

Guiding you from sample to biology

Our experts are ready to help you with your phosphoproteomics project. Ideally, we set up an online meeting where we get to learn about your project. Here, we discuss the different options to reach your aim.

Get an price estimate within 24 hours

If you need an estimate for your project, we are happy to help. We encourage you to request an initial free consultation to assess which approach best fits your phosphor research aim

By booking a meeting, you agree to our privacy policy.

Sample preparation and Mass Spectrometry

Phosphorylation using mass spectrometry

The proteomics laboratory specializes in handling your specimens from sample preparation to LC-MS/MS with high quality. It ensures a robust and reproducible result, which is essential for future follow-up studies.

Experienced professionals

Biogenity’s team and its partners works with the highest standards to ensure the best quality handling and analysis of your samples. We collaborate with carefully selected proteomics laboratories for phosphoproteomics, ensuring that every aspect of the workflow is executed by specialized personnel.

The latest equipment from Thermo Fisher Scientific

We pride ourselves on being able to offer the latest equipment on the market. Our collaborating proteomics laboratory has the latest equipment from Thermo Fisher Scientific, ensuring a deep coverage. If you’d like to see the complete platform list, follow this link.

Up to 15,000 phosphorylations per sample

If you need high accuracy with TMT labeling for global and targeted post-translational modification, the accurate Orbitrap Eclipse Tribrid or the super fast Exploris 480 is the ideal solution. We are happy to guide you around the various options when it comes to equipment.

Data analysis, including report, figures, and tables

Visualization of your phosphoproteomics

Data analysis is where you get the overview of your animal-based analysis. You will receive an easy-to-follow report describing the data analysis and results hereof, step-by-step, together with a folder containing all tables and figures. To ensure that you have the material needed, we always provide a vast number of figures and tables.

Let us be your data scientist

Biogenity’s data analysis is much more than simply a data analysis. It offers an insight into what biology is, e.g., potentially activated or deactivated in the different conditions, without the hassle of postprocessing the data. Our team does it all: data filtration, outlier testing, variance plotting with PCA, clustering, and the statistics - and that is just with the statistical data analysis.

Download DEMO

Measure how your protein changes over time

Phosphoproteomics is particularly well suited for time-course experiments measuring the signaling occurring within minutes of signal initiation, as phosphorylation occurs on a sub-minute timescale.

Compared to the traditional proteomic approach of measuring the baseline differences between two conditions, phosphorylation benefits from multiple temporal measurements to establish the cascades of kinase and phosphatase activation.

Our phosphoproteomics expert will help design your experiment, ensuring that we answer your specific research questions at hand.

Bioinformatical data analysis of phosphoproteomics

Bioinformatics allows for much deeper data analysis of your phosphorylated proteins to identify pathways, biological processes, molecular functions, cellular components, and kinases that are changing in your data. The bioinformatic data analysis includes enrichment analysis, comparative figures, and network mapping.

Interactive plots to highlight signaling pathways

With our bioinformatic data analysis, you will also get interactive plots that allow you to explore the signaling pathways that are enriched in your data. It lets you take the regulated proteins into perspective, showing which biological pathways or biological processes are potentially up or down-regulated. This helps you get a good understanding of what is happening in the different conditions.

Delivery and discussion

Delivered directly to your inbox

When your phosphoproteomics analysis is ready, we deliver it directly to your inbox. Your data and reports are easy to access and secured with encryption and password protection.

You can receive your sample leftovers sent by courier, or Biogenity can safely destroy them after project completion. It is entirely up to you.

Plant a tree with Biogenity and One Tree Planted

Partnering with One Tree Planted, we want to work towards a brighter future with better treatment for diseases and a healthy planet to enjoy life. For us, our children, grandchildren, and the generations to come.

Biogenity donates a dozen trees for every project delivered to support local communities, reforestation in the Amazon rainforest, and reduce CO2 emissions. See how we are making a difference together.

Post-consultation and discussion

It is important to us that you get the most out of your experiment, which is why we always offer a post-consultation where you can ask all your questions about the results. Together, we ensure that you have the best prerequisite for your further research. The consultation can also be used to explore the next step, whether you are looking to expand the project with additional omics studies or validate the results.

What you can expect:

  • Explanation of the different steps in the sample analysis
  • Discussion of the data
  • Explanation of the different steps in the data analysis
  • Q&A to the report

Other proteomics LC-MS/MS approaches



Biogenity can provide you with up to 2,000 proteins in your study if you need a high through-put proteomic service with deep coverages.

Learn more


If you are looking for a deep proteome characterization of tissue, Biogenity has tailored workflows for your research.

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Processes regulated by phosphoproteomics

Phosphoproteomics regulates many cellular processes and functions, like cell growth, cell mobility, transcription, signaling pathways, protein degradation, PTM cross-talk, and more. We have listed all the processes and functions regulated by phosphorylation:

Processes regulated by phosphorylation

  • Transcription
  • Cell growth
  • Cell cycle
  • Apoptosis
  • Cell mobility
  • Cytoskeletal reorganization
  • Carcinogenesis
  • Cell adhesion
  • Differentiation
  • Translation
  • Translation
  • DNA repair
  • Signaling pathways
  • Autophagy
  • Chromatin organization
  • Endocytosis
  • Exocytosis
  • RNA splicing
  • RNA stability
  • Neural plasticity
— Show more —

Functions regulated by phosphorylation

  • Molecular association
  • Activity
  • Intracellular localization
  • PTM cross-talk
  • Protein degradation
  • Protein stabilization
  • Protein conformation
  • Receptor sensitivity and recycling
  • Protein processing
— Show more —