Phosphoproteomics Analysis

Precision Proteomics Services


At Biogenity, we use phosphoproteomics to map dynamic signaling events, revealing active kinases, pathways, and regulatory mechanisms central to your biological question.

Phosphoproteomics is excellent for measuring cell signalling pathways. By applying mass spectrometry to your phosphorylated peptides, Biogenity can detect which proteins, kinases, and pathways are active, thus, identifying which proteins are involved in the biological processes pertaining to your experimental question.

If you’d like to learn more, we are happy to discuss your project and help you get the most out of your phosphoproteomics analysis.


Let’s discuss your project


Up to 15,000
phosphorylations per sample


TMT labeling
on Orbitrap Eclipse Tribrid


Time-course designs
signaling within minutes

Guiding you from sample to biology

At Biogenity, we understand that phosphoproteomics research is a sophisticated field that demands precision, expertise, and a tailored approach. Our team of proteomics experts is dedicated to guiding you through every step of your study, ensuring your project not only succeeds but also contributes to your scientific venture.



Get a free consultation

We invite you to an online consultation, during which we can address your questions and offer an expert assessment of how our phosphoproteomics strategies can be customized to meet your research goals.


Sample preparation and sample analysis

Phosphorylation using mass spectrometry

Our proteomics laboratory specializes in handling your specimens from sample preparation to LC-MS/MS with high quality. It ensures a robust and reproducible result, which is essential for future follow-up studies.

Biogenity’s team works with the highest standards to ensure the best quality handling and analysis of your samples. We ensure that every aspect of the workflow is executed by specialized personnel.

Up to 15,000 phosphorylations per sample

If you need high accuracy with TMT labeling for global and targeted post-translational modification, the accurate Orbitrap Eclipse Tribrid or the super fast Exploris 480 is the ideal solution. We are happy to guide you around the various options when it comes to equipment.

Enrichment of the phosphorylated peptides before LC-MS/MS gives you deep coverage of phosphorylation sites, and we help you choose between the Orbitrap Eclipse Tribrid and the Exploris 480 depending on your study design.

Data analysis, including report, figures, and tables

Analysis of your phosphoproteomics data

Data analysis is where you get your overview. You will receive an easy-to-follow report describing the data analysis of your phosphoproteomics samples and results hereof, step-by-step, together with a folder containing all tables and figures. To ensure that you have the material needed, we always provide a vast number of figures and tables.



Measure how your protein changes over time

Phosphoproteomics is particularly well suited for time-course experiments measuring the signaling occurring within minutes of signal initiation, as phosphorylation occurs on a sub-minute timescale.

Compared to the traditional proteomic approach of measuring the baseline differences between two conditions, phosphorylation benefits from multiple temporal measurements to establish the cascades of kinase and phosphatase activation.

Delivery and discussion

Delivery, post-consultation and discussion

At the conclusion of our phosphoproteomics analysis, we ensure a seamless transition from discovery to application. We deliver detailed reports and data, followed by a post-delivery consultation to discuss your study's insights and findings.


What to expect during post-consultation:

  • Explanation of the different steps in the sample analysis
  • Discussion of the data
  • Explanation of the different steps in the data analysis
  • Q&A to the report(s)

Together, we ensure that you have the best prerequisite for your further research. The consultation can also be used to explore the next step, whether you are looking to expand the project with additional omics studies or validate the results.


Get Started Today