Ubiquitination

Proteome analysis


Protein ubiquitination is involved in nearly all processes in eukaryotic cells. Proteomics using mass spectrometry enables the global unbiased identification of ubiquitinated residues on proteins. This is crucial to understanding the ubiquitination landscape of your samples. Biogenity can get you the needed overview in your experiments, from the K48-linked chains that signal protein degradation to cell fate decisions of K63-linked ubiquitin chains.

If you’d like to learn more, we are happy to discuss your project and help you get the most out of your ubiquitination analysis.


Let’s discuss your project

Guiding you from sample to biology

Our experts are ready to help you with your ubiquitination research study. Ideally, we set up an online meeting where we get to learn about your project. Here, we discuss the different options to reach your aim.



Get a price estimate for your project

If you need an estimate for your project, we are happy to help. We encourage you to request an initial free consultation to assess which ubiquitination proteomics approach fits your research aim.



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Sample preparation and Mass Spectrometry

Ubiquitination using mass spectrometry

The proteomics laboratory specializes in handling your specimens from sample preparation to LC-MS/MS with high quality. It ensures a robust and reproducible result, which is essential for future follow-up studies.



Experienced professionals

Biogenity’s team and its partners work with the highest standards to ensure the best quality handling and analysis of your samples. We collaborate with carefully selected proteomics laboratories for the analysis of ubiquitination, ensuring that every aspect of the workflow is executed by specialized personnel.

The latest mass spectrometers for ubiquitination

We pride ourselves on offering some of the best equipment on the market. Our collaborating proteomics laboratory has some of the latest equipment from Thermo Fisher Scientific, ensuring a deep coverage. If you’d like to see the complete platform list, follow this link.



State-of-the-art insight

Working with a specialized laboratory gives you state-of-the-art results and provides you with a unique insight into the ubiquitination landscape. Contact us and learn about Biogenity's available methods and how the analysis result will fit your research aim.

The various research aims can result in various challenges. Having different instruments allows for tailoring the instrument's use to your need. Whether you are looking for high sensitivity and accuracy to detect small changes in low abundant proteins or looking for budget-friendly methods for less complex samples, we have you covered.

Data analysis, including report, figures, and tables

Visualisation of your protein ubiquitination analysis

Data analysis is where you get the overview of your ubiquitination analysis. You will receive an easy-to-follow report describing the data analysis and results hereof, step-by-step, together with a folder containing all tables and figures. To ensure that you have the material needed, we always provide a vast number of figures and tables.



Explore the ubiquitin code in your samples

The immense complexity of ubiquitin modifications is uniquely suited to large-scale proteomic analysis. When using the ubiquitin-specific diglycine remnant on ubiquitinated lysines, proteomics is able to accurately determine the specific lysine residue that is ubiquitinylated in your samples.

Identify the regulated ubiquitinated proteins

With accurate and quantitative residue mapping, Biogenity can identify the regulated ubiquitinated proteins in your samples. This enables you to increase the depth of your understanding through bioinformatic analysis, allowing you to investigate the pathways, biological processes, molecular functions, cellular components, and E3 ligases that are enriched in your data. The bioinformatics data analysis includes enrichment analysis, comparative figures, and network mapping.



Interactive plots to highlight signaling pathways

With our bioinformatic data analysis, you will also get interactive plots that allow you to explore the signaling pathways that are enriched in your data. It lets you take the regulated proteins into perspective, showing which biological pathways or biological processes are potentially up or down-regulated. This helps you get a good understanding of what is happening in the different conditions.

Delivery and discussion

Delivered directly to your inbox

When your ubiquitination analysis is ready, we deliver it directly to your inbox. Your data and reports are easy to access and secured with encryption and password protection.

You can receive your sample leftovers sent by courier, or Biogenity can safely destroy them after project completion. It is entirely up to you.



Plant a tree with Biogenity and One Tree Planted

Partnering with One Tree Planted, we want to work towards a brighter future with better treatment for diseases and a healthy planet to enjoy life. For us, our children, grandchildren, and the generations to come.

Biogenity donates a dozen trees for every project delivered to support local communities, reforestation in the Amazon rainforest, and reduce CO2 emissions. See how we are making a difference together.

Post-consultation and discussion

It is important to us that you get the most out of your experiment, which is why we always offer a post-consultation where you can ask all your questions about the results. Together, we ensure that you have the best prerequisite for your further research. The consultation can also be used to explore the next step, whether you are looking to expand the project with additional omics studies or validate the results.

What you can expect:

  • Explanation of the different steps in the sample analysis
  • Discussion of the data
  • Explanation of the different steps in the data analysis
  • Q&A to the report

Other proteomics LC-MS/MS approaches

Discovery-based proteomics

Proteomics

Biogenity offers global protein analysis of your samples, getting deep proteome coverages with state-of-the-art accuracy.

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Phosphoproteomics

Proteomics

Biogenity can provide you with deep coverage and an easy-to-follow report if you are interested in kinase activity and phosphorylation patterns.

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Peptidomics

Proteomics

Discover the identity biomarkers, mutation, degradation by-products, allergens, and epitopes with peptidomics.

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